We have the necessary technologies and instrumentation to support fine structural Protein Characterization and Peptide Characterization programs, as well as the characterization of liposomal products.

Technological platforms for Protein Characterization include:

• LC-MS/MS (Ion Trap) for amino acid sequence and primary structure analysis/confirmation
• Electron Transfer Dissociation (ETD) for analysis of Post-Translational Modifications (PTM), including glycosylation, sulfation, and Phosphorylation
• LC-MS (ESI-TOF) in conjunction with enzymatic digestion to determine disulfide bridge mapping
• Various modes of spectroscopy for protein secondary structure analysis
• Automated Edman Degradation for N-Terminal sequence analysis
• HPLC in combination with enzymatic digestion and fluorescence detection for oligosaccharide profiling and quantitation of sialylation
• Peptide Mapping
• Amino Acid Compositional Analysis (AAA)
• ELISA/Immunoassay method development and validation for protein/peptide quantification and residual contaminant analysis
• SDS-PAGE, IEF and 2-D Gel Electrophoresis coupled with Densitometry
• Determination of Extinction Coefficient
• SEC for aggregation and oligomerization analysis
• RP-HPLC for purity and impurity analysis

These Protein Characterization methodologies may be applied to products such as: Recombinant and Fusion Proteins, Antibody Products, Recombinant Vaccine Products, Peptide Based Therapeutics, Liposomal Formulations, Glycoprotein products, etc.